| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chen, Min-syun (12)
|
ADSARY |
35.51 |
37.42 |
1.91 |
| 2 |
Tandlmayer, Elizabeth A (12)
|
ADSSCY |
39.64 |
38.80 |
-0.84 |
| 3 |
Mienaltowski, Allison E (11)
|
ADDACC |
40.43 |
40.05 |
-0.38 |
| 4 |
Frisoni, Olympia S (12)
|
ADGLEN |
40.71 |
40.58 |
-0.13 |
| 5 |
Gilbert, Kendall G (11)
|
ADGLEN |
41.97 |
42.10 |
0.13 |
| 6 |
Mohamed, Amber H (11)
|
ADSSCY |
42.45 |
42.97 |
0.52 |
| 7 |
Ormsby, Mikayla (12)
|
ADGLEN |
43.61 |
43.48 |
-0.13 |
| 8 |
Hurff, Natasha B (11)
|
ADSARY |
47.10 |
43.75 |
-3.35 |
| 9 |
Kreig, Hannah (12)
|
ADGLEN |
43.87 |
43.76 |
-0.11 |
| 10 |
Bodien, Julia M (11)
|
ADSSCY |
43.20 |
44.58 |
1.38 |
| 11 |
Boomhower, Brianna E (11)
|
ADSSCY |
45.61 |
44.88 |
-0.73 |
| 12 |
Schager, Kierra G (12)
|
ADDACC |
47.31 |
48.11 |
0.80 |
| 13 |
Trahan, Ella L (11)
|
ADSARY |
46.99 |
48.22 |
1.23 |
| 14 |
Dupuree, Mikayla (11)
|
ADGLEN |
48.49 |
48.48 |
-0.01 |
| 15 |
Zebrowski, Olivia G (12)
|
ADSARY |
50.39 |
49.80 |
-0.59 |
| 16 |
Thompson, Madison C (12)
|
ADSARY |
49.91 |
51.30 |
1.39 |
| 17 |
Trautner, Magdalene Anne B (11)
|
ADSARY |
52.37 |
51.44 |
-0.93 |
| 18 |
Johnson, Stephanie P (11)
|
ADSSCY |
56.35 |
55.09 |
-1.26 |
|
Dunn, Kaylie A (11)
|
ADDACC |
|
DQ |
- |
|
Krein, Ava (11)
|
ADGLEN |
|
DQ |
- |
|
Thrall, Lydia B (12)
|
ADSARY |
42.94 |
NS |
- |
|
Goffioul, Clara Marie A (11)
|
ADSARY |
50.36 |
NS |
- |
|
Albee, Rowan (11)
|
ADDACC |
52.05 |
NS |
- |